Postdoctoral researchers
We have openings for outstanding scientists who are excited about our research and have a strong background in biochemistry, structural (cryo-EM, x-ray), molecular or cell biology.
Please email Bob for more information.
Department of Biochemistry & Molecular Biology
Gordon Center for Integrative Science Room W238
University of Chicago
929 East 57th Street
Chicago, IL 60637
lab: (773) 834.2675
office: (773) 834.2292
fax: (773) 834.5416
email: bkeenan@uchicago.edu
AFFILIATIONS
Department of Biochemistry & Molecular Biology
Chemistry & Biology Interfaces
UChicago Advanced Electron Microscopy facility
Keenan Lab Publications
Substrate-driven assembly of a translocon for multipass membrane proteins
Sundaram, A.#, Yamsek, M.#, Zhong, F.#, Hooda, Y., Hegde, R.S. and Keenan, R.J. (2022)
Nature, in press. (#=equal contribution) (doi: 10.1038/s41586-022-05330-8)
Mechanism of an intramembrane chaperone for multipass membrane proteins
Smalinskaitė, L.#, Kim, M.K.#, Lewis, A.J.O., Keenan, R.J., and Hegde, R.S. (2022)
Nature, in press. (#=equal contribution) (doi: 10.1038/s41586-022-05336-2)
The mechanisms of integral membrane protein biogenesis
Hegde, R.S. and Keenan, R.J. (2021) Nature Reviews Molecular Cell Biology. (doi: 10.1038/ s41580-021-00413-2)
An ER translocon for multi-pass membrane protein biogenesis
McGilvray PT, Anghel SA, Sundaram A, Zhong F, Trnka MJ, Fuller JR, Hu H, Burlingame AL, Keenan RJ. (2020)
eLife, Aug 21;9:e56889. (doi: 10.7554/eLife.56889)
The architecture of EMC reveals a path for membrane protein insertion
O'Donnell JP, Phillips BP, Yagita Y, Juszkiewicz S, Wagner A, Malinverni D, Keenan RJ, Miller EA, Hegde RS. (2020)
eLife, May 27;9:e57887. (doi: 10.7554/eLife.57887)
A structural perspective on tail-anchored protein biogenesis by the GET pathway
Mateja, A and Keenan, RJ (2018) Current Opinion in Structural Biology, 51:195-202.
(doi: 10.1016/j.sbi.2018.07.009)
Identification of Oxa1 homologs operating in the eukaryotic endoplasmic reticulum
Anghel, S.A., McGilvray, P.T., Hegde, R.S., Keenan, R.J. (2017) Cell Reports, 21:3708-3716.
(doi: 10.1016/j.celrep.2017.12.006)
Tail-anchored protein insertion by a single Get1/2 heterodimer
Zalisko, B.E., Chan, C., Denic, V., Rock, R.S. and Keenan, R.J. (2017) Cell Reports, 20:2287-2293.
(doi: 10.1016/j.celrep.2017.08.035)
Msp1 is a membrane protein dislocase for tail-anchored proteins
Wohlever ML, Mateja A, McGilvray PT, Day KJ and Keenan, RJ (2017) Molecular Cell, 67:194-202.
(doi: 10.1016/j.molcel.2017.06.019)
The GET system inserts the tail-anchored SYP72 protein into endoplasmic reticulum membranes
Srivistava R, Zalisko BE, Keenan RJ, Howell SH. (2017) Plant Physiol., 173:1137-1145.
(doi: 10.1104/pp.16.00928)
Ubiquilins chaperone and triage mitochondrial membrane proteins for degradation
Itakura, E., Zavodszky, E., Shao, S., Wohlever, M.L., Keenan, R.J., Hegde, R.S. (2016) Molecular Cell, 63:21-33.
(doi: 10.1016/j.molcel.2016.05.020)
Data publication with the structural biology data grid supports live analysis
Meyer PA, Socias S, Key J, Ransey E, Tjon EC, Buschiazzo A, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Anderson KS, Baxter RH, Blacklow SC, Boggon TJ, Bonvin AM, Borek D, Brett TJ, Caflisch A, Chang CI, Chazin WJ, Corbett KD, Cosgrove MS, Crosson S, Dhe-Paganon S, Di Cera E, Drennan CL, Eck MJ, Eichman BF, Fan QR, Ferré-D'Amaré AR, Fromme JC, Garcia KC, Gaudet R, Gong P, Harrison SC, Heldwein EE, Jia Z, Keenan RJ, Kruse AC, Kvansakul M, McLellan JS, Modis Y, Nam Y, Otwinowski Z, Pai EF, Pereira PJ, Petosa C, Raman CS, Rapoport TA, Roll-Mecak A, Rosen MK, Rudenko G, Schlessinger J, Schwartz TU, Shamoo Y, Sondermann H, Tao YJ, Tolia NH, Tsodikov OV, Westover KD, Wu H, Foster I, Fraser JS, Maia FR, Gonen T, Kirchhausen T, Diederichs K, Crosas M, Sliz P. (2016) Nature Commun., 7:10882. (doi: 10.1038/ncomms10882)
Conformational chaperones for structural studies of membrane proteins using antibody phage display
Dominik, P.K., Borowska, M.T., Dalmas, O., Kim, S.S., Perozo, E., Keenan, R.J., Kossiakoff, A.A. (2016) Structure,
24:300-309. (doi: 10.1016/j.str.2015.11.014)
A dual fluorescent reporter for the investigation of methionine mistranslation in live cells
Gomes A.C., Kordala A.J., Strack R., Wang X., Geslain R., Delaney K., Clark W.C., Keenan R.J., Pan T. (2016) RNA,
22:467-476. (doi: 10.1261/rna.054163.115)
A YidC-like protein in the archaeal plasma membrane
Borowska, M.T., Dominik, P.K., Anghel, S.A., Kossiakoff A.A., Keenan R.J. (2015) Structure, 23:1715-1724.
(doi: 10.1016/j.str.2015.06.025)
Structure of the Get3 targeting factor in complex with its membrane protein cargo
Mateja A, Paduch M, Chang H.Y., Szydlowska A, Kossiakoff A.A., Hegde R.S., Keenan R.J. (2015) Science,
347:1152-1155. (doi: 10.1126/science.1261671)
Fission yeast profilin is tailored to facilitate actin assembly by the cytokinesis formin Cdc12
Bestul, A.J., Christensen, J.R., Grzegorzewska, A.P., Burke, T.A., Sees, J.A., Carroll, R.T., Sirotkin, V., Keenan, R.J.,
Kovar, D.R. (2015) Mol Biol Cell. 26:283-93. (doi: 10.1091/mbc.E13-05-0281)
Tail-anchored membrane protein insertion into the endoplasmic reticulum
Hegde, R.S. and Keenan, R.J. (2011) Nature Reviews Molecular Cell Biology, 12:787-798. (doi: 10.1038/nrm3226)
The mechanism of membrane-associated steps in tail-anchored protein insertion
Mariappan, M.#, Mateja, A.#, Dobosz, M., Bove, E., Hegde, R.S., Keenan, R.J. (2011) Nature, 477:61-66.
(doi: 10.1038/nature10362) (#=co-first authors)
A conserved archaeal pathway for tail-anchored membrane protein insertion
Sherrill, J., Mariappan, M., Dominik, P., Hegde, R.S., Keenan, R.J. (2011) Traffic, 12:1119-1123.
(doi: 10.1111/j.1600-0854.2011.01229.x)
Noncytotoxic DsRed derivatives for whole-cell labeling
Strack R.L., Keenan R.J., Glick B.S. (2011) Methods Mol Biol., 699:355-70.
A ribosome-associating factor chaperones tail-anchored membrane proteins
Mariappan M, Li X, Stefanovic S, Sharma A, Mateja A, Keenan R.J., Hegde R.S. (2010) Nature, 466:1120-4.
Chromophore formation in DsRed occurs by a branched pathway
Strack, R.L., Strongin, D.E., Mets, L., Glick, B.S., Keenan, R.J. (2010) J. Am. Chem. Soc., 132:8496-8505.
The structural basis of tail-anchored membrane protein recognition by Get3
Mateja, A., Szlachcic, A., Downing, M.E., Dobosz, M., Mariappan, M., Hegde, R.S., Keenan, R.J. (2009) Nature,
461:361-366. (doi:10.1038/nature08319)
A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling
Strack, R.L., Hein, B., Bhattacharyya, D., Hell, S.W., Keenan, R.J., Glick, B.S. (2009) Biochemistry, 48:8279-8281.
Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling
Strack, R.L., Bhattacharyya, D., Glick, B.S., Keenan, R.J. (2009) BMC Biotech., 9:32.
A noncytotoxic DsRed variant for whole-cell labeling
Strack, R.L., Strongin, D.E., Bhattacharyya, D., Tao, W., Berman, A., Broxmeyer, H.E., Keenan, R.J., Glick, B.S. (2008)
Nature Methods, 5:955-957.
Spectral diversity of fluorescent proteins from the anthozoan Corynactis californica
Schnitzler, C.E., Keenan, R.J., Matysik, A., Christianson, L.M., McCord, R., Haddock, S.H.D. (2008) Marine Biotech.,
10:328-342.
Structural rearrangements near the chromophore influence the maturation speed and brightness of
Strongin, D.E., Bevis, B., Khuong, N., Downing, M.E., Strack, R.L., Sundaram, K., Glick, B.S., Keenan, R.J. (2007)
Protein Eng. Des. Sel., 20:525-534.
The molecular basis of glyphosate resistance by an optimized microbial acetyltransferase
Siehl, D.L., Castle, L.A., Gorton, R., Keenan, R.J. (2007) J. Biol. Chem., 282:11446-11455.
DNA Shuffling as a tool for protein crystallization
Keenan, R.J., Siehl, D.L., Gorton, R., Castle, L.A. (2005) Proc. Natl. Acad. Sci. USA, 102:8887-8892.
Evolution of a microbial acetyltransferase for modification of glyphosate: a novel tolerance strategy
Siehl, D.L., Castle, L.A., Gorton, R., Chen, Y.H., Bertain, S., Cho, H.J., Keenan, R.J., Liu, D., Lassner, M.W. (2005)
Pest Manag. Sci., 61:235-40.
Laboratory-Directed Protein Evolution
Yuan, L., Kurek, I., English, J., Keenan, R.J. (2005) Microbiol. Mol. Biol. Rev., 69:373-392.
Nontransgenic crops from transgenic plants
Keenan, R.J., Stemmer, W.P. (2002) Nature Biotech., 20:215-216.
The Signal Recognition Particle
Keenan, R.J., Freymann, D.M., Stroud, R.M., Walter, P. (2001) Annu. Rev. Biochem., 70:755-775.
Perspectives: structural biology. SRP – where the RNA and membrane worlds meet
Walter, P., Keenan, R.J., Schmitz, U. (2000) Science, 287:1212-1213.
Structure of the phylogenetically most conserved domain of SRP RNA
Schmitz, U., Behrens, S., Freymann, D.M., Keenan, R.J., Lukavsky, P., Walter, P., James, T.L. (1999) RNA,
5:1419-1429.
Functional changes in the structure of the SRP GTPase on binding GDP and Mg2+-GDP
Freymann, D.M., Keenan, R.J., Stroud, R.M., Walter, P. (1999) Nature Struct. Biol., 6:793-801.
Crystal Structure of the Signal Sequence Binding Subunit of the Signal Recognition Particle
Keenan, R.J., Freymann, D.M., Walter, P., Stroud, R.M. (1998) Cell, 94:181-191.
Structure of the conserved GTPase domain of the signal recognition particle
Freymann, D.M., Keenan, R.J., Stroud, R.M., Walter, P. (1997) Nature, 385:361-364.
NMR studies of the most conserved RNA domain of the mammalian signal recognition particle (SRP)
Schmitz, U., Freymann, D.M., James, T.L., Keenan, R.J., Vinayak, R., Walter, P. (1996) RNA, 2:1213-1227.
Rutenber, E., Fauman, E.B., Keenan, R.J., Fong, S., Furth, P.S., Ortiz de Montellano, P.R., Meng, E., Kuntz, I.D.,
DeCamp, D.L., Salto, R., Rosé, J.R., Craik, C.S., Stroud, R.M. (1993) J. Biol. Chem., 286:15343-15346.
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